Draft Genome Sequence of Lactobacillus mucosae CRL 573, a mannitol producer bacterium

BLECKWEDEL, JULIANA; TERAN, LUCRECIA C.; BONACINA, JULIETA; SAAVEDRA, LUCILA; MOZZI, FERNANDA; RAYA, RAÚL

Rosario

Congreso; 50th Annual Meeting; 2014

Argentine Society for Biochemistry and Molecular Biology

Mannitol is a polyol that has multiple industrial applications due to its interesting physicochemical properties, being mainly used as natural sweetener in the food industry. It could be applied as a food additive or be produced in situ by food?grade microorganisms leading to the elaboration of naturally mannitol-enriched foods. Lactobacillus mucosae CRL 573 was selected for its ability to efficiently produce mannitol from fructose. Here we present the draft genome of CRL 573 sequenced using the whole-genome shotgun strategy (Ion Torrent personal genome machine). Initial assemblies resulted in a 2.26 Mbp draft genome with 38 contigs and overall GC content of 46.6%. This size and the GC content are comparable with those of the lactic acid bacteria (LAB) group. Genome annotation was done using the Rapid Annotations using Subsystems Technology server and a total of 2,715 coding sequences and 157 of RNAs were predicted. CRL 573 was found to contain a putative mannitol?dehydrogenase (mdh) gene, with a genomic context similar to the one present in Lb. reuteri CRL1101, that confirms its ability to produce mannitol. Furthermore, the analysis allowed us to identify a threonine?DH?like domain typical of the NAD(H)?dependent alcohol dehydrogenase family (MDR).