Conjugated biolipid production by bifidobacterium strains with potential health promoting properties

TERÁN, V.;; VINDEROLA, G.;; LUNA PIZARRO, P.; RODRIGUEZ, J.M.; RAYA, R.; MEDINA, R.; VAN NIEUWENHOVE, C.

Rosario

Congreso; SAIB; 2014

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Conjugated linoleic (CLA) and linolenic (CLNA) acids are, respectively isomers of linoleic and linolenic acid that exhibit health-promoting properties such as anticarcinogenic, antiatherogenic, anti-inflammatory and antidiabetic activities. Bacteria able to synthetize them are considered suitable for the development of dairy products or as probiotic strains for in situ bioproduction. The aim of this work was to investigate CLA/CLNA production of 8 bifidobacteria strains cultured in MRS-cys and skim milk with LA and LNA. After 48 h-37ºC under anaerobic conditions, lipid were extracted and analyzed by UV method followed by GC. Results showed that all strains were able to form CLA and/or CLNA in both medium with percentages of conversion from 1-14% for CLA and 1-65% for CLNA in MRS-cys broth. In skim milk percentages were from 1-12% for CLA and 1-96% for CLNA. B. animalis ssp. lactisINL2 showed the highest production of c9,t11,c12-CLNA (MRS-cys) and B. breveZL1228 resulted the best producer of t9,t11-CLA (skim milk). Growth compatibility assays between these two selected strains were performed. The relatedness between INL2 and commercial probiotic strains was determined by pulse field gel electrophoresis. These results suggest that INL2 and ZL1228 strains are suitable candidates for the design of new fermented dairy products naturally containing high concentrations of bioactive lipids.