Physicochemical conditions and culture media design to optimize the production of pharmacologically active metabolites for chronic wounds.

RAMOS AN; SESTO CABRAL ME; LINDON S.; CRUZ, M.E.; GONZALEZ SN; VALDEZ JC

Córodoba

Congreso; III Reunión Internacional de Ciencias Farmacéuticas RICiFa; 2014

Facultad de Ciencias Químicas - UNC

Lactobacillus plantarum culture supernatants (LAPS) have antimicrobial, pro‐healing and anesthetic properties, so our medical team applied it in chronic wounds with very encouraging results. The composition of LAPS was determined and its action mechanism was established. The metabolites responsible for the properties act synergistically, which positions the complex mixture of LAPS as a single active pharmaceutical ingredient (API). However, the need for personalized treatments to suit different types of chronic wounds led to devise and develop this study. Based on this, the aim of this work was to design methods and culture media that increase the therapeutic effectiveness of the supernatants. Multiple modifications were made in MRS broth composition and L. plantarum was cultivated in the resulting media with different physicochemical conditions to obtain modified supernatants (LAPSm). The bacteriostatic, bactericidal and anti-biofilm activity (crystal violet) of LAPSm was tested on four strains typically isolated from chronic wounds (Pseudomonas aeruginosa, Staphylococcus aureus, S. epidermidis and Serratia marcescens). The concentration of barbiturates in LAPSm was quantified by GCMS and its relative anesthetic potency in healthy volunteers was evaluated. The LAPSm obtained from media with high concentrations of yeast extract (0.4%) possessed the greatest barbiturates concentration and maximum anesthetic power. Those containing greater amount of meat extract (0.1%), cations (Na, K and Mg salts 0.3%) and surfactants (Tween 80 0.4%) had the highest capacity of biofilm disruption. When the glucose (0.3%) and galactose (0.3%) concentration were increased in media, the LAPSm had the greatest bacteriostatic and bactericidal power as well as those grown with higher concentrations of CO2 (10%). Finally, media with boric acid (0.1%) had higher inhibitory capacity of biofilm formation. The results obtained in this work will allow in the future a LAPSm manufacture with a greater therapeutic effectiveness and even custom properties for each type of wound.