Use of probiotic fermented milk in an ovoalbumin allergy model applied to young mice

VELEZ EVA; MALDONADO GALDEANO, CAROLINA; BIBAS BONET, EUGENIA; PERDIGÓN, GABRIELA.

Mar del Plata

Congreso; LIX reunion Cientifica anual de la Sociedad Argentina de Investigación Clínica (SAIC). LXII Reunión Anual de la Sociedad de Inmunología (SAI); 2014

Sociedad Argentina de Inmunologia

When used in adult mice, probiotic fermented milk (PFM) lowers anti-OVA IgE in serum and BAL increasing a-OVA IgG and regulating Th1/Th2 balance towards a Th1 response with high level of IFN-γ. We observed that PFM does not lowers IgE production in young mice. The aim of this work was to explore if PFM prevents allergy development using other regulatory process. Young BALB/c mice (21 days old) were split into 5 groups: Normal-Control(NC), Basal(B-5days-PFM), OVA-Sensitization-Control(SC), Previous feeding and sensitization(P-5d-PFM+OVA+H2O) and Continuous feeding(C-5d-PFM+OVA+PFM). At 7 and 15 days-post-sensitization (dPS) and 2 days-post-re-stimulus (dPR) mice were sacrificed and samples of: serum, BAL, small intestinal fluid (SIF) and tissue and lungs tissue were taken to determine: anti-OVA IgE, IgG, IL-4, IL-10, IgA + cells and inducible Treg cells (lungs). We found that a-OVA IgE and IgG increased gradually reaching a peak at 2 dPR in all groups. In BAL, a-OVA IgG also increased at 2dPR; a-OVA IgE was no detectable. In serum IL-4 was lower in C group compared to SC at 15 dPS and 2 dPR (p