Anti-inflammatory ability of plant derived immunobiotic Lactobacillus delbrueckii subsp. delbrueckii 4408L and its immunogenic extracellular polysaccharides via regulating TLR signaling in porcine intestinal epitheliocytes

PAULRAJ KANMANI; SATOSHI WACHI; YOHSUKE TOMOSADA; TOSHIHITO YURI; HISAKAZU KOBAYASHI; SHINTARO EGUSA; TOMOYUKI SHIMAZU; YOSHIHITO SUDA; HISASHI ASO; KIMIKO TSUZUKI; TADAO SAITO; JULIO VILLENA; TAKASHI MISHIMA; HARUKI KITAZAWA

Boston

Congreso; 114th General Meeting of the American Society of Microbiology; 2014

American Society of Microbiology

Immunobiotics are known to modulate intestinal immune responses by regulating toll-like receptors (TLRs) signaling pathways, which are responsible for the induction of cytokines in response to microbial associated molecular patterns. However, little is known about the immunomodulatory activity of compounds from immunobiotics. Previously, we reported that porcine intestinal epithelial (PIE) cells are effective tools for the selection of immunobiotic bacteria, and they also could be of value for evaluating immunobiotics-derived compounds. Therefore, the aims of this study were: i) to find whether immunobiotic and its immunogenic compounds modulate the response of PIE cells against enterotoxigenic Escherichia coli (ETEC) and, ii) to gain insight into the molecular mechanisms involved in the anti-inflammatory effects immunobiotic and its immunogenic compounds by evaluating the role of TLR2 TLR4 and TLR negative regulators in the modulation of pro-inflammatory cytokine production, activation of mitogen-activated protein kinase (MAPK) and nuclear factor kB (NF-kB) pathways and in PIE cells.