Relevance of IFN gamma; and IL10 induced by probiotic fermented milk administration in the regulation of allergies development.

VELEZ EVA; BIBAS BONET, EUGENIA; CARMUEGA, ESTEBAN; WEILL, RICARDO; PERDIGÓN, GABRIELA; MALDONADO GALDEANO, CAROLINA

Carovigno

Workshop; 7th International Immunonutrition Workshop; 2014

Different studies have shown the ability of probiotics to reduce and regulate immune response during the allergic development processes. The aim of this work was to evaluate the potential efficacy of a probiotic fermented milk (PFM) in the prevention of allergy development and to investigate the mechanisms involved in a mouse model. Ovoalbumin (OVA) sensitized adult BALB/c mice were fed previous (before sensitization) or continuously with a probiotic fermented milk. Groups: Normal-Control (NC), Basal (B-5days-PFM); OVA-Sensitization-Control (SC), Previous-(P-5d-PFM+OVA+H2O) and Continuous-(C-5d-PFM+OVA+PFM) treatment. At 7 and 15 days post-sensitization (dPS) and 2 days post-re-stimulus (dPR) we studied in bronchoalveolar lavage fluid (BALF): specific-IgE, IgG, total-S-IgA. In macerated lung tissue: IL10, IL4, INFγ. In serum: specific-IgE, IgG, IgG1, IgG2a, IL4 and IL10; were determined by ELISA. In lung tissue IL4+, IL10+, INF-γ+, CD4+, CD8+ and F4/80+ cells were evaluated by immunofluorescence. Results showed that PFM significantly reduced specific IgE in serum and BALF at 7 dPS (p≤0.05) without altering the specific response to a-OVA-IgG and total S-IgA for 7 dPS and 2dPR. The main cytokines up-regulated by PFM were IL10 (7dPS) and INF-γ (7 and 15 dPS, and 2DPR) (p≤0.05) with a switch from Th2 to a Th1 response. This effect could also be due to the induction of Treg cells in lungs. The use of functional foods like PFM seems to be a promising strategy in the prevention or treatment of allergy, decreasing the IgE response but not a-OVA-IgG.