Cytokine expression in stressed mice fed with probiotic strains.

PALOMAR, MARTÍN; PERDIGÓN, GABRIELA; MALDONADO GALDEANO, CAROLINA

Mar del Plata

Congreso; LIX reunion Cientifica anual de la Sociedad Argentina de Investigación Clínica (SAIC). LXII Reunión Anual de la Sociedad de Inmunología (SAI); 2014

Sociedad Argentina de Inmunologia

The gastrointestinal tract and the immune system are sensitive to different stressor factors. In previous works, stressed mice model, showed changes in the intestinal microbiota composition and in the number of IgA-producing cells that was reverted by probiotics administration. The aim of this work was to determine the cytokine producing cells in the intestinal lamina propria of the small intestine,in intestinal fluid (IF), serum (S) and supernatant of intestinal epithelial cells (IECs), peritoneal macrophages (PMQ) and spleen macrophages (SMQ) of mice in the stress model and the changes induced by probiotcs administration. BALB/c mice were stressed over 11 days in a stress protocol consisting of food deprivation during 12h/day (8:00 pm-8: 00am) and diurnal immobilization for 3h. Mice were divided into 8 groups: Normal Control (NC), NC feed Lactobacillus casei CRL431 (NC+CRL431), NC feed Lactobacillus paracasei CNCMI-1518 (NC+ CNCMI-1518), NC feed with probiotic fermented milk (NC+PFM), stressed mice (S+H2O),stressed mice feed Lactobacillus casei CRL431 (S+CRL431), stressed mice feed Lactobacillus paracasei CNCMI-1518 (S+ CNCMI-1518), and stressed mice feed with probiotic fermented milk (S+PFM). Samples of serum, intestinal fluid, IECs, PMQ and SMQ were taken at 12 day. The IL-10 production was measured by ELISA test and the cytokine+ cells (IL-1, IL-6, IL-10 andTNFα) were determined in intestinal tissue section by indirect immunofluorescence. Results show that probiotic strains and probiotic fermented milk restore cytokines+ cells to NC levels in gut of stressed mice (p≤0.01). IL-10 levels is decreased in stressed mice, the probiotic administration restored this values compared to NC (p≤0.05).The PMQ and SMQ supernatant not showed significant changes. Conclusion: The consumption of probiotics restore the cytokines levels to normal values, in stressed mice, improving the gut mucosal immune system.