CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Probiotic fermented milk in an ovoalbumin allergy model applied to young mice
Autor/es:
VELEZ, E.M.; MALDONADO C.; BIBAS BONET, M.E.; PERDIGÓN, G.
Lugar:
Mar del Plata - Buenos Aires - Argentina
Reunión:
Congreso; LIX Reunión Científica Annual de la Sociedad Argenitna de Investigación Clínica (SAIC) ? LXII Reunión Anual de la Sociedad Argentina de Inmunología; 2014
Institución organizadora:
SAI - SAIC
Resumen:
When used in adult mice, probiotic fermented milk (PFM) lowers anti-OVA IgE in serum and BAL increasing a-OVA IgG and regulating Th1/Th2 balance towards a Th1 response with high level of IFN-γ. We observed that PFM does not lowers IgE production in young mice. The aim of this work was to explore if PFM prevents allergy development using other regulatory process. Young BALB/c mice (21 days old) were split into 5 groups: Normal-Control(NC), Basal(B-5days-PFM), OVA-Sensitization-Control(SC), Previous feeding and sensitization(P-5d-PFM+OVA+H2O) and Continuous feeding(C-5d-PFM+OVA+PFM). At 7 and 15 days-post-sensitization (dPS) and 2 days-post-re-stimulus (dPR) mice were sacrificed and samples of: serum, BAL, small intestinal fluid (SIF) and tissue and lungs tissue were taken to determine: anti-OVA IgE, IgG, IL-4, IL-10, IgA + cells and inducible Treg cells (lungs). We found that a-OVA IgE and IgG increased gradually reaching a peak at 2 dPR in all groups. In BAL, a-OVA IgG also increased at 2dPR; a-OVA IgE was no detectable. In serum IL-4 was lower in C group compared to SC at 15 dPS and 2 dPR (p≤0.05) but had no differences between groups in SIF. IL-10 showed significant increase only in P group at 7 and 15 dPS of the studied periods in serum; in SIF IL-10 was significantly lower in P and C compared to SC group (p≤0.05). Treg cells expression was not affected by PFM intake or sensitization with OVA. PFM does not exert the same effect observed previously in adult mice. It does not lowers a-OVA IgE and showed no other regulatory effects according to the levels of IL-10/IL-4 (in those mice that consumed PFM) and the lack of expression of Treg cells in sensitized treated and non treated groups