EFFECT OF ORAL ADMINISTRATION OF LACTIC ACID

M. RUSSO, E. FABERSANI, M. C. ABEIJON MUKDSI, S. GONZALEZ, P. GAUFFIN CANO, R. B.MEDINA

San Miguel de Tucumán

Simposio; IV International Symposium on Lactic Acid Bacteria: Food, Health and Applications; 2013

Caloric restriction (CR) can be defined as undernutrition without malnutrition. CR regimen provides essential nutrients and vitamins, but limits the total calorie intake. CR represents an ideal model to study how changes in nutritional status influence on metabolic parameters. Human intestinal bacteria with feruloyl esterase (FE) activity have the ability to generate ferulic acid with antioxidant properties in vivo. Administration of lactic acid bacteria (LAB) with FE activity can reverse metabolic markers present in animal models of metabolic syndrome. However, there is no information about the effect of FE?]producing LAB administration in animal models of CR and its relationship with the host health. The aim of the present work was to evaluate the effect of LAB adminsitration on intestinal FE activity and metabolic parameters in mice fed a CR diet. Balb/c mice were daily fed a restricted amount of food during 45 days in order to reach a mild malnutrition (CR group). Control mice received a standard diet (Control group). Two LAB supplemented CR groups were included: I) Lb group, administered with 108 cfu/day/mouse of Lact. fermentum CRL1446 (with FE activity), and II) Lc group, administered with Lac. lactis CRL1434 (without FE activity) at the same dose. Animals were sacrified at day 1, 20, and 45. FE activity was determined in gut content and mucose extract using methylferulate as substrate. Released ferulic acid was detected by HPLC. Animal metabolic status was evaluated by determination of plasmatic glucose concentration and lipid profile. Results showed a 1.3?]fold decrease in total intestinal FE activity in CR group, compared to control group at day 45. Lb group showed similar activity at day 20 and 1.25?]fold higher activity at day 45, compared to control group. In Lc group, activity was similar to control group throughout the administration period. Both Lb and Lc groups showed significantly higher total intestinal FE activity than CR group. Plasmatic glucose, triglyceride and colesterol levels in animals from CR group were significantly lower than in control group. At day 45, Lb group showed lower glucose levels than CR group.  Regarding total cholesterol, both treated groups showed lower levels than CR group, higher reduction being observed in mice from Lb group. No differences in triglycerides levels were observed among CR and treated groups throughout the trial. Results showed that oral administration of L. fermentum CRL 1446 to animals fed a caloric restriction diet increases intestinal FE activity, reaching values similar or even higher than those from control group, favoring the bioavailability of antioxidant free ferulic acid. Moreover, it showed the improvement of metabolic and oxidative status.