Immune response after weaning, in mice fed with a probiotic fermented milk and Sensitized with ovoalbumin

VELEZ EVA; BIBAS BONET, EUGENIA; CARMUEGA, ESTEBAN; WEILL, RICARDO; PERDIGÓN, GABRIELA; MALDONADO GALDEANO, CAROLINA

Córdoba

Congreso; LVIII Reunión Científica Anual Sociedad Argentina de Investigación Clínica (SAIC)-LX Reunión Anual Sociedad Argentina de Inmunología (SAI).; 2013

Previously, we studied in adult mice the effect of probiotic fermented milk (PFM) administration when animals are sensitized with OVA. We found that PFM lowers the production of anti OVA (a-OVA) IgE without altering normal systemic and mucosal immune response in lungs or small intestines. Aim: to determine in young animals (21 days old) exposed to OVA if the PFM administration exerts similar effect to that found in adult mice. 21 days old BALB/c mice were split into 5 groups: Normal-Control(NC), Basal(B-5days-PFM), OVA-Sensitization-Control(SC), Previous feeding and sensitization(P-5d-PFM+OVA+H2O) and Continuous feeding(C-5d-PFM+OVA+PFM) treatment. At 7 and 15 days post sensitization (dPS) and 2d post-re-stimulus (dPR) we studied in serum and BAL a-OVA IgE and IgG, total (T) S-IgA and IL4; in small intestinal fluid (SIF) T-S-IgA and IL4. Results: in P and C a-OVA IgE and IgG were near toSC levels at 7-15 dPS. For 2dPR we observed an increase in all studied groups (p≤0.05). In SIF at 15dPS T-S-IgA showed significant decrease in SC (p≤0.05), P and C remained similar toNC and B. In BAL all sensitized groups had a slight increase in T-S-IgA compared to NC and B. IL4 was increased in SIF from SC and P at 7dPS and for 15dPS in C group (p≤0.05), no differences were found at 2dPR. In BAL P and C showed a high level of IL4 only at 15dPS (p≤0.05). PFM showed that systemic immunity was stimulated with high levels of a-OVA IgE and IgG, with no effect in the levels T-S-IgA. We believe that results obtained were due to the immature Immune System and that PFM at this stage does not influence the regulatory effect observed in adult mice with regard to the IgE