EFFECT OF A PROBIOTIC FERMENTED MILK IN AN OVER EXPOSITION OF THE ANTIGEN OVOALBUMIN IN A MOUSE ALLERGY MODEL

VELEZ EVA; BIBAS BONET, EUGENIA; CARMUEGA, ESTEBAN; WEILL, RICARDO; PERDIGÓN, GABRIELA; MALDONADO GALDEANO, CAROLINA

Vancouver

Congreso; 16th International Congress of Mucosal Immunology.; 2013

Sociedad Internacional de Inmunidad de Mucosas

Previously we found that probiotic fermented milk (PFM) containing Lc-DN114001 administration regulates IgE production. We analyzed the immune response in lungs when mice are re-stimulated with ovoalbumin (OVA). Groups: Normal-Control (NC), Basal (B-5days-PFM); OVA-Sensitization-Control (SC), Previous-(P-5d-PFM+OVA+H2O) and Continuous-(C-5d-PFM+OVA+PFM) treatment. At 7 and 15d post-sensitization (dPS) and 2d post-re-stimulus (dPR) we studied in BAL-fluid: specific-IgE, IgG, total-S-IgA, IL10, IL4, INFin lungs tissue: IL4+, IL10+ cells, and in serum: IL4, specific-IgG1 and IgG2a. Specific-IgE was significantly reduced in P and C treatment at 7dPS but not after OVA-re-stimulation, total-specific-IgG was increased in P and C at 2dPR. IgG2a (Th1-balance) was significantly higher in P and C at 7dPS compared to SC and to IgG1. IFN increased only in C at 7 and 15dPS. IL10 increased in C at 7dPS. Total-S-IgA increased in C at 7dPS. IL4+cells were augmented in SC. PFM was only effective in the primary response to IgE by IL10 production favoring Th1-response, without suppressing S-IgA and with regulated levels of IFN until 15dPS in C group