CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Assessement of preservation methods applied to dairy and cutaneous propionibacteria with industrial relevance
Autor/es:
NEME SCHEIJ L.; PÉREZ CHAIA A. Y ZÁRATE, G.
Lugar:
San Miguel de Tucumán
Reunión:
Congreso; XXIX Jornadas Científicas de la Asociación de Biología de Tucumán; 2013
Institución organizadora:
Asociaciación de BIología de Tucumán
Resumen:
Propionibacteria are divided into dairy  and cutaneous based on habitat and are used as cheese starters, nutraceutical producers and probiotics. Industrial application of microorganisms greatly relies on preservation methods that guarantee cultures with long term functionality. Our objective was to assess the effect of different preservation methods on viability and stability during storage of 2 dairy and cutaneous strains of propionibacteria. P. freudenreichii and P. avidum strains were harvested at stationary phase (1010 CFU/mL) and preserved by hypobiotic: storage under mineral oil and anabiotic methods: freezing at -20¨¬C and lyophilization with/without crioprotectors. Samples were stored during 9 months at 4¨¬C and the number of viable cells, re-activation in fresh medium and cellular damage (release of proteins, nucleic acids and ¥â-gal) were determined at intervals. The strains survived to treatments and long-term storage. After 9 months, 104 CFU/mL and 109 CFU/g were recovered under the best conditions of hypobiotic and anabiotic methods respectively. Glycerol, DMSO and LEL were successful protectors and suspension media for propionibacteria preserved by freezing whereas glutamate and trehalose were the best protectors for lyophilized cells. The protectors assayed also prevented cell inactivation and damage caused by preservation.