Effects of lactic acid bacteria on leptin secretion in murine adipocytes.

FABERSANI, E; GONZALEZ, S; GAUFFIN CANO, PAOLA

Tucumán

Simposio; IV SIMPOSIO INTERNACIONAL DE BACTERIAS LACTICAS; 2013

Leptin, a pleiotropic hormone mainly produced by the adipose tissue, regulates multiple homeostatic functions such as food intake, reproductive and immune functions, besides basal metabolism. The objective of this study was to evaluate the influence of different lactic acid bacteria (LAB) on leptin and cytokine secretion by murine adipocyte cells. These parameters and the Obr- leptin receptor expression were also evaluated in co-cultures of adipocytes and macrophage cells in order to select a microorganism with low pro-inflammatory potential, able to positively and negatively modulate leptin secretion. Bacterial cell suspensions of 14 LAB strains were used (Lactobacillus casei CRL431,  Lactobacillus acidophilus CRL258,  Lactobacillus acidophilus CRL1063,  Lactobacillus casei CRL66, Lactobacillus casei CRL72, Lactobacillus casei CRL117, Lactobacillus fermentum CRL1446, Lactococcus lactis CRL1434, Lactobacillus plantarum CRL350, Lactobacillus plantarum CRL352, Lactobacillus plantarum CRL353, Lactobacillus plantarum CRL355, Lactobacillus paracasei CRL575 y Lactobacillus casei ssp rhamnosus CRL576). The influence of different LAB on immunological properties (cytokine) and Obr receptor expression in RAW 264.7 macrophage cell line was evaluated. These macrophages were stimulated with bacterial cell suspensions (1x107 UFC/mL) at 37°C under 5% CO2  for 24 h. Adipocytes isolated from Balb/c mice were stimulated with LAB suspensions (1x107 UFC/mL) under the same conditions, and adipokines levels were determined. For co-culture experiments, adipocytes were cultured with Raw 264.7 macrophage cells (5x104 cels/mL, 1:1). Leptin, TNF-á, IL-6, IL-10 and MCP-1 levels were determined in the culture supernatants by ELISA test. The principal component analysis (PCA) allow the classification into three distinctive groups of LAB according to their ability to modulate both leptin and leptin receptor expression, and inflammatory profile. We selected one strain from each group in order to perform further studies: 1) Lactobacillus casei CRL431 (low leptin levels, medium inflammatory profile and ObR expression), Lactobacillus fermentum CRL1446 (medium leptin levels, low inflammatory profile and ObR expression) and Lactococcus lactis CRL1434 (high leptin levels, medium-high inflammatory profile and high ObR expression). Our results suggested that different strains may have different functional roles and applications in diverse pathologies. Therefore, modulation of circulating leptin levels may be considered as a promising novel strategy to intervene on different nutritional conditions (obesity and undernutrition).