CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Influencia de la administración oral de lactobacilos sobre la actividad esterasa intestinal en un modelo murino de restricción calórica.
Autor/es:
RUSSO, M; FABERSANI, E; ABEIJÓN MUKDSI, C; GONZALEZ, S; GAUFFIN CANO, PAOLA; MEDINA, R
Lugar:
Tucumán
Reunión:
Simposio; IV SIMPOSIO INTERNACIONAL DE BACTERIAS LÁCTICAS; 2013
Resumen:
Caloric restriction (CR) can be defined as undernutrition
without malnutrition. CR regimen provides essential nutrients and vitamins, but
limits the total calorie intake. CR represents an ideal model to
study how changes in nutritional status influence on metabolic parameters. Human
intestinal bacteria with feruloyl esterase (FE) activity have the ability to
generate ferulic acid with antioxidant properties in vivo. Administration of lactic acid bacteria (LAB) with FE
activity can reverse metabolic markers present in animal models of metabolic
syndrome. However, there is no information about the effect of FE-producing LAB
administration in animal models of CR and its relationship with the host health. The aim of the present work was to evaluate the effect of LAB
adminsitration on intestinal FE activity and metabolic parameters in mice fed a
CR diet. Balb/c mice were daily fed a restricted amount of food during 45 days
in order to reach a mild malnutrition (CR group). Control mice received a
standard diet (Control group). Two LAB supplemented CR groups
were included: I) Lb group, administered with 108 cfu/day/mouse of Lact. fermentum CRL1446 (with FE
activity), and II) Lc group, administered
with Lac. lactis CRL1434 (without FE
activity) at the same dose. Animals were sacrified at day 1, 20, and 45. FE
activity was determined in gut content and mucose extract using methylferulate
as substrate. Released ferulic acid was detected by HPLC. Animal metabolic status
was evaluated by determination of plasmatic glucose concentration and lipid
profile. Results showed a 1.3-fold decrease in total intestinal FE activity in
CR group, compared to control group at day 45. Lb group showed similar activity at day 20 and
1.25-fold higher activity at day 45, compared to control
group. In Lc group, activity was similar to control group throughout
the administration period. Both Lb and Lc groups showed significantly higher
total intestinal FE activity than CR group. Plasmatic glucose, triglyceride and
colesterol levels in animals from CR group were significantly lower than in control
group. At day 45, Lb group showed lower glucose levels than CR group. Regarding
total cholesterol, both treated groups showed lower levels than CR group,
higher reduction being observed in mice from Lb group. No differences in
triglycerides levels were observed among CR and treated groups throughout the
trial. Results showed that oral administration of L. fermentum CRL 1446 to animals fed a caloric restriction diet increases
intestinal FE activity, reaching values similar or even higher than those from control
group, favoring the bioavailability of antioxidant free ferulic acid. Moreover,
it showed the improvement of metabolic and oxidative status.