CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Immunobiotic evaluation of functional feed materials in porcine intestinal immune and epithelial cells
Autor/es:
NORI KUMAGAE; YOHSUKE TOMOSADA; JULIO VILLENA; TAKUYA TAKAHASHI; SYOICHI HOSOYA; HISASHI ASO; TADAO SAITO; SASAKI T; YOSHIDA M; TANABE H; HARUKI KITAZAWA
Lugar:
Liepzig
Reunión:
Congreso; 5th Congress of European Microbiologists ? FEMS2013; 2013
Institución organizadora:
European Microbiologists Societies
Resumen:
ckground: The establishment of drug-free feeding system has been required for secure and healthy livestock production. Although functional feed materials containing microorganisms as drug alternatives that may enhance intestinal immunity are expected to be beneficial for reducing diarrhea caused by pathogenic bacterial infection in weaned piglets, the effects of such materials on the porcine intestine have not been investigated in detail. Objectives: We evaluated the effects of microbial feed materials on the anti-inflammatory and immunoregulatory functions in porcine intestinal immune and epithelial cells to select the beneficial materials for young pigs. Methods: Six feed materials containing Aspergilli, Lactobacilli and Bifidobacteria were used. The immune cells isolated from Peyer ́s patches and mesenteric lymph nodes from porcine ileum were stimulated with test materials, and then evaluated for lymphocyte mitogenicity and the induction of cytokine gene expressions. In addition, porcine intestinal epithelial cells pre-stimulated with test materials were treated with heat-killed enterotoxigenic Escherichia coli (ETEC), and analyzed for the inhibition of proinflammatory cytokine expressions. Results and conclusions: The feed material containing Bifidobacteria significantly augmented lymphocyte mitogenicity. This material also induced relatively higher expression of IL-2, IL-6 and IFN-gamma in the immune cells, inhibited the ETEC-induced overexpression of IL-6 and IL-8. These results suggest that this feed material stimulates intestinal epithelial and immune cells to exert anti-inflammatory and immunoregulatory effects, suggesting that this material is expected to contribute to the healthy growth of young pigs without using antimicrobial growth promoters.