CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Modulación de la respuesta inmune mucosa por la fracción no-bacteriana deleche fermentada por L. helveticus R389
Autor/es:
VINDEROLA, CELSO GABRIEL; MATAR, CHANTAL; PALACIOS, JORGE; PERDIGON, GABRIELA
Lugar:
San Miguel de Tucumán, Argentina
Reunión:
Simposio; II Simposio Internacional de Bacterias Lacticas. Primer encuentro Red BAL Argentina; 2006
Institución organizadora:
CEREL-CONICET
Resumen:
The health promoting effects ascribed to probiotic bacteria and fermented dairy products arise not only from bacteria themselves but also from metabolites (exopolysaccharides and peptides). The aim of this work was to study the gut mucosa immunomodulation capacity of the non-bacterial fraction of the milk fermented by Lactobacillus helveticus R389 at constant pH 6 (NBFpH6). The effects on IL-6 production by small intestine epithelial cells, the profile of IgA+ and cytokine+ cells (IL-2, IL-6, IL-10, TNF-a and IFN-g) induced in the gut lamina propria, and the levels of total and specific secretory IgA in the lumen of BALB/c mice that received NBFpH6 for 2, 5 or 7 days were examined. There was an increase in the number of IgA+, IL-10+, IL-2+ and IL-6+ cells after all feeding periods. Total S-IgA in the small intestine lumen increased in mice that received NBFpH6 for 2 days. However, no specific antibodies against NBFpH6 were detected. Feeding of NBFpH6 for 7 days (P < 0.05) significantly enhanced IL-6 secretion by small intestine epithelial cells. NBFpH6 induced a non-specific mucosal response that was and down regulate for protective immunity, enhancing IL-6 production by epithelial cells and IgA production in the small intestine. These events improve the immunological defenses at the intestinal level, increasing host protection against pathologies. Because mucosal immune responses induced by certain dietary antigens play a large part in the prevention of gastrointestinal diseases, the oral administration of a mucosal adjuvant such as NBFpH6 may positively affect the milieu of the intestinal lumen. The opportunity exists then to manipulate the constituents of the lumen of the intestine through dietary means, thereby enhancing the health condition of the host.