CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Lipid rafts: target for probiotic lactic acid bacteria?
Autor/es:
RODRIGUEZ AV
Lugar:
San Miguel de Tucum¨¢n, Argentina
Reunión:
Simposio; II International Symposium on Lactic Acid Bacteria. First Argentinean Lab Net Meeting; 2006
Institución organizadora:
CERELA-CONICET
Resumen:
LIPID RAFTS: TARGET FOR PROBIOTIC LACTIC ACID BACTERIA?
Rodriguez AnaV.
Centro de Referencia para Lactobacilos (CERELA). Chacabuco 145. 4000-Tucum¨¢n, Argentina. E-mail: anavirr@cerela.org.ar
Cholesterol and sphingolipids-enriched membrane microdomains of eukaryotic cells or lipid rafts have been well studied as surface cell site used by several pathogen microorganisms, microbial-derived toxins, virus and parasite to interact, bind or enter into host cells. For instance, rafts may provide a mechanism by which receptors are concentrated and thereby promote binding. Interestingly, multimeric toxins were found to associate with rafts not to concentrate the toxin, but to offer clusters of receptors. One well-known example is the cholera toxin that interacts with ganglioside GMI; clusters of GMI are concentrated in rafts allowing high affinity binding of cholera toxin by a ¡°Velcro¡± type mechanism. Rafts were also found to be important for intracellular survival of bacteria themselves like E. coli, P. aeruginosa, Mycobacterium spp. and others. Even the growing evidence that several microbes specifically target rafts domains, no interactions between lipid rafts and no pathogen bacteria have been reported. By studying the role of these microdomains in bacterial pathogenesis, we may be able to get a better understanding of disease mechanisms and hopefully design more effective treatment strategies. In the same way, investigating the role of rafts in probiotic effects of lactic acid bacteria (LAB) could allow us to better understand the beneficial mechanisms of LAB. We present here the first evidences of two probiotic LAB, L. acidophilus CRL1014 and L. reuteri CRL 1098, which modified TNF-¦Á production by human cells with disrupted rafts.