INFLUENCE OF LACTIC ACID BACTERIA ON SERUM LEPTIN SECRETION AND IMMUNE PROPERTIES IN MICE

GAUFFIN CANO, PAOLA; ABEIJÓN MUKDSI, C; GONZALEZ, S

San Miguel de Tucumán

Congreso; VII Congreso Argentino de Microbiología General "Samige del Bicentenario"; 2011

The leptin plays multiple homeostatic functions in the organism. The information about the influence of lactic acid bacteria (LAB) on the leptin secretion is scarce and contradictory. Aim: The objective of this study was to compare the influence of different doses of LAB oral administration on leptin secretion and its relationship with immunological parameters in mice. Materials and methods: Adult Swiss mice received during 5 consecutive days, 108 ufc/ml of Lactobacillus casei CRL 431 (Lc431); and 106, 107, and 108 ufc/ml of Lactobacillus plantarum 236 (Lp236) and Lactobacillus rhamnosus ETC14 (ETC14) administered in drinking water. Serum leptin concentration, number of IgA+ B lymphocytes in mucosal small intestine and percentage of phagocytic activity were determined. Results: We observed a significant increase on serum leptin after Lc431 and Lp236 administration, whereas ETC14 induced a significant decrease of this hormone. No differences between doses of the same microorganism were observed. We only observed significant increase of peritoneal phagocitosis with Lc431 administration. When we analyzed count of number of IgA+ cells, we viewed that 108 ufc/ml of Lc431, and 106 ufc/ml of ETC14 induced significant increases of these cells. While different doses of Lp236 did not induce changes in IgA+ cells. These results suggest that the oral administration of LAB has influence in serum leptin secretion. This effect was strain dependent. The modulation of leptin secretion in addition to the immunomodulatory properties of some lactic acid bacteria may be part of the criteria of selection for probiotic microorganisms. We suggest as new potential probiotic Lactobacillus rhamnosus ETC14.