Casein hydrolysate generated by the proteinase of Lactobacillus delbrueckii subsp lactis CRL 581 prevents experimental colitis in mice.

ESPECHE TURBAY, B.; DE MORENO DE LEBLANC, A.; MOZZI, F.; PERDIGON, G.; SAVOY DE GIORI, G.; HEBERT, E. M.

Egmond aan Zee

Congreso; 10th Symposium on Lactic Acid Bacteria. Celebrating 30 years of LAB research.; 2011

Federation of European Microbiological Societies and Netherlands Society for Microbiology (FEMS)

Lactobacillus delbrueckii subsp. lactis CRL 581 releases a series of potentially bioactive peptides (antihypertensive and phosphopeptides) from alfa- and beta-caseins. In this study, the potential immunomodulatory properties of the bioactive peptides released by CRL 581 cells from casein were assayed using a trinitrobenzene sulfonic acid (TNBS)-induced a colitis murine model. The casein hydrolysate was obtained by incubation of non-proliferating CRL 581 cells with casein for 4 h at 40C. In a preventive study, BALB/c mice received orally casein, the hydrolysate or PBS buffer daily for 10 days before colitis. TNBS was administered at day 11 and mice were sacrified at 0, 3 and 7 days after TNBS. Inflammation was assessed by measuring changes in body weight, mortality, microbial translocation, histology of the intestine, myeloperoxidase (MPO) and beta-glucuronidase activities, and cytokines (IL-10, IFN) in intestinal fluid. The administration of the hydrolysates produced less weight loss and mortality compared to the control of inflammation. In the preventive assay, the values of beta-glucuronidase and MPO activities as well as liver translocation were lower compared to the levels observed in the TNBS group. These observations were correlated with the histological features of the intestine. Also, an increase of the regulatory cytokine IL-10 and a decreased in the proinflammatory cytokine IFN were observed in the animals which received the hydrolysates. These results suggest that L. delbrueckii subsp. lactis CRL 581 inoculated in milk is able to generate peptides with anti-inflammatory activity in vivo. The industrial importance and the potential beneficial health properties of the bioactive peptides released by CRL 581 cells from milk proteins make this microorganism a promissory strain for the production of novel functional foods