CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Differential pattern of response to Lactobacillus reuteri CRL 1098 between two in vitro eukaryotic cell culture systems: relevance on probiotic strain selection with specific applications
Autor/es:
1. JUAREZ, GE;; MECHOUD, MA; FONT DE VALDEZ, G; RODRIGUEZ, AV
Lugar:
San Miguel de Tucumán
Reunión:
Congreso; VII Congreso Argentino de Microbiología General, Sociedad Argentina de Microbiología General (SAMIGE).; 2011
Institución organizadora:
SAMIGE
Resumen:
Differential pattern of response to Lactobacillus reuteri CRL 1098 between two in vitro eukaryotic cell culture systems: relevance on probiotic strain selection with specific applications Probiotics are live microorganisms which when administered in adequate amounts confer health benefits to the host. Among these beneficts, probiotics are able to modulate immune responses and they have been extensively studied as immunomodulatory agents in the fields of inflammatory diseases. Certain strains from Lactobacillus and Bifidobacterium genera have the capacity to modify the profile of pro- and anti-inflammatory mediators released from immune cells in in vitro and in vivo inflammation experimental models. The aim of this work was to compare the capacity of L. reuteri CRL 1098 to exert an anti-inflammatory effect in two cellular in vitro models: human peripheral blood mononuclear cells (PBMC) and mouse macrophage cell line (RAW 264.7). L. reuteri or its supernatant were incubated for 4 and 24 h with control or LPS-stimulated PBMC and RAW 264.7 cells at 37 °C and 5% CO2. TNF-α, IL-10 and nitric oxide (NO) producion was then measured in the co-cultures supernatants. The results showed that after 4h incubation L. reuteri and its supernatant diminished TNF-α production by 33 and 34%, respectively in control PBMC and by 68 and 37%, respectively in LPS-stimulated PBMC. In RAW 264.7 cells L. reuteri increased 147 times the TNF-α production while L. reuteri supernatant had no effect on TNF-α production by control and LPS-stimulated RAW 264.7. NO production decreased by effect of L. reuteri supernatant similarly than TNF-α after 4 and 24h incubation in both LPS-stimulated PBMC and RAW 264.7. In contrast, L.reuteri or its supernatant did not affect the IL-10 production by PBMC and RAW 264.7 cells in control and LPS-stimulated cells. Our findings prove that a single bacterial strain can generate different immune patterns of responses depending on which in vitro model was evaluated. Therefore, anti-inflammatory properties of L. reuteri CRL 1098 might be tested in a number a cell cultures before extrapolation to in vivo trials in an experimental animal model.