Caracterización de la microbiota intestinal de heces de niños eutróficos y aislamiento de cepas de lactobacilos con potencial probiótico

LORENZO PISARELLO, MARÍA JOSÉ; VINTIÑI ELISA; GONZALEZ SILVIA; MARCELA MEDINA

Tafí del Valle

Jornada; XXVIII Jornadas Científicas de la Asociación de Biología de Tucumán; 2011

Asociación Argentina de Biología

The intestinal microbiota (IM) is a complex microenvironment and includes a variety of bacterial genera and species among which the Lactobacilli are one of the main genera used as probiotics.Objective:To characterize the major bacterial genera present in the IM of eutrophic children and isolate strains of Lactobacillus for potential use as probiotics in human health. Stool of 15 healthy children were analyzed.MM:1)Count by conventional techniques: total aerobes and anaerobes,Enterobacteriaceae and lactobacilli.The N° of bacteria was determined by plate count and the results were expressed as CFU/g of feces, 2) Isolation of lactobacilli and evaluation of their morphology (GRAM stain), specific biochemical properties (API system), resistance to acid pH and bile. The results are expressed as mean total counts x g of faeces: Anaerobes: 2x109 CFU/g,Aerobes: 5x109 CFU/g, Enterobacteriaceae: 2x106 CFU /g and Lactobacillus: 3x106 CFU/g. It were isolated 2 strains of lactobacilli that with high resistance to acid pH and bile, fundamental requirements of a strain, to be defined as a probiotic. Further studies are necessary to their potential use in the development of products for preventive applications and/or particular therapeutic