HAEMOSTATIC ALTERATIONS IN INFECTED MALNOURISHED MICE: EFFECT OF NASAL TREATMENT WITH Lactobacillus casei

ZELAYA, H; LAIÑO, J; HARO, C; AGÜERO, G

Tafí del Valle. Tucumán

Jornada; XXVIII Jornadas Científicas de la Asociación de Biología de Tucumán; 2011

Asociación de Biología de Tucumán

Aim. To evaluate the preventive effect of different doses of Lactobacillus casei (Lc) intranasally administered, on haemostatic alterations induced by a septic process in malnourished mice. Malnourished mice (MN) received 7d balanced diet (BD) or BD with intranasal addition of different doses of Lc during the last 2d (BD+Lc107, BD+Lc108, BD+Lc109 cells/d/mice). Experimental, MN and Wellnourished (WN) groups were infected with Streptococcus pneumoniae. Malnourishment altered serum total proteins (TP) and haemostatic parameters. Renutrition with Lc improved them without significant (p<0.05) difference between doses. After infection, pathogen was detected in lung and blood. Mice treated with Lc had negative hemocultures and lower bacterial counts at 240 h post infection (hpi), BD+Lc109 showed significant lower counts in lung. The infection altered Prothrombin Time, Activated Partial Thromboplastin Time, Fibrinogen, Platelet count and TP in all groups. MN showed the highest changes. Renutrition with Lc had beneficial effects, BD+Lc109 showed better behavior (TP12hpi WN= 59.70±2.88 g/L; MN= 40.00±2.88; BD= 53.80±2.16; BD+Lc107= 46.20±1.70; BD+Lc108= 43.00± 1.67; BD+Lc109= 62.10±2.69. Intranasal addition of 109 cells/d/mice of Lc was the most effective doses to favor pathogen depuration and recover some haemostatic parameters.