CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Toll-like receptor-2 activating bifidobacteria strains regulate inflammatory responces in porcine intestinal epithelial cells
Autor/es:
HITOMI FUJIE; JULIO VILLENA; MASANORI TOHNO; KYOKO MORIE; TOMOYUKI SIMAZU; HISASHI ASO; YOSHIHITO SUDA; TAKESHI SHIMOSATO; NORISUKE IWABUCHI; JIN-ZHONG XIAO; KEIJI IWATSUKI; TADAO SAITO; MUNEO NUMASAKI; HARUKI KITAZAWA
Lugar:
Geneva
Reunión:
Congreso; 4th Congress of European Microbiologists; 2011
Resumen:
The selection of immunobiotic tolerogenic bifidobacteria could contribute to the development of new immunologically functional foods that can be used to prevent inflammatory intestinal disorders, in both humans and animals. The objectives of this study were to select potential immunobiotic bifidobacteria by using several immunoassay systems and to elucidate the immunological mechanisms behind their beneficial effect. Immunomodulatory bifidobacteria were selected by using a combination of two methods: the NF-B reporter assay using porcine toll-like receptor 2-expressing transfectant (HEKpTLR2 immunoassay system) and the mitogenic assay using porcine immunocompetent cells from Peyer’s patches (PP). In addition, we evaluated the anti-inflammatory activity of the pre-selected strains by using a porcine intestinal epithelial (PIE) cell line and an inflammatory challenge with enterotoxigenic Escherichia coli (ETEC). Among the four pre-selected strains by the combination of the immunoassay systems, Bifidobacterium breve MCC-117 (Bb117), the strain with the highest HEKpTLR2 and mitogenic activities, was able to most efficiently modulate inflammatory response triggered by ETEC in PIE cells. By using PIE cells and swine PP immunocompetent cell co-culture system, we demonstrated that the immunoregulatory effect of Bb117 was related to the capacity of the strain to influence PIE and PP immune cell interactions, leading to the stimulation of regulatory T cells. These results suggest that bifidobacteria that possess high activity in both the HEKpTLR2 immunoassay system and the mitogenic assay would be expected as a potential anti-inflammatory strains. In addition, our findings provide a scientific rationale for the use of Bb117 to prevent ETEC induced intestinal inflammation.