The nasal administration of Lactobacillus rhamnosus promotes the recovery of lung B cells affected by malnutrition

NATALIA PAOLA BARBIERI; SUSANA SALVA; HÉCTOR MATÍAS HERRERA; JULIO VILLENA; SUSANA ALVAREZ

Mar del Plata

Congreso; LVIII Reunión Anual de la Sociedad Argentina de Inmunología XIII Jornada Científica del Grupo Rioplatense de Citometría de Flujo 3º Jornadas Argentinas de Inmunodeficiencias Primarias (SAP); 2010

Sociedad Argentina de Inmunología

The nasal administration of Lactobacillus rhamnosus CRL1505 (Lr05) is able to improve the innate and specific immune responses against Streptococcus pneumoniae in malnourished mice. In this work, we analyzed the effect of the nasal administration of Lr05 on the recovery of lung B cells during a repletion treatment. Weaned mice were malnourished after consuming a protein-free diet (PFD) for 21d. Malnourished mice were repleted with a balanced diet (BD) during 7d or BD 7d with Lr05 nasal administration (108cells/mouse/d) on d 6 and 7 (BD+Lr05). The malnourished control mice (MC) received PFD while the well-nourished control group (WC) consumed BD. At the end of the treatments, we evaluated: body weight, total number of lung cells and the B cell population of lung by flow cytometry (CD19, B220, HSA, IgM, IgD). The MC mice showed lung cell counts significantly lower than the WC. Both repletion treatments increased lung cells but only the BD+Lr05 mice reached higher values than the WC group (p