EFFECT OF PREVENTIVE ADMINISTRATION OF LACTIC ACID BACTERIA ON THE NUMBER AND ACTIVITY OF MYELOID CELLS AFTER CYCLOPHOSPHAMIDE TREATMENT

VILLENA JULIO; SALVA SUSANA; HERRERA MATÍAS; BARBIERI NATALIA; KITAZAWA HARUKI; ALVAREZ SUSANA

Kosice-Slovakia

Congreso; International Scientific Conference on Probiotics and Prebiotics - IPC2010; 2010

The Scientific Committee of IPC2010

The daily changes in the number and activity of mature granulocytes and progenitor cells in blood and bone marrow (BM), after an immunosuppression treatment with cyclophosphamide (Cph), were evaluated in mice. In addition, we studied the influence of preventive treatments with lactic acid bacteria (LAB) on the recovery of myeloid cells. Swiss-albino mice were fed Lactobacillus casei CRL431 (Lc431, 109 cells/day/mouse) or Lactobacillus rhamnosus CRL1506 (Lr06, 108 cells/day/mouse) for 2 or 5 consecutive days respectively. After each treatment, these groups and untreated controls (CG) received an intraperitoneal (ip) injection of Cph (150 mg/Kg), and were sacrificed at different times post-chemotherapy (for 15d). Total and differential counts, Gr-1 and CD34 expression and peroxidase activity (Px+) of blood and BM cells were studied. Moreover, peritoneal macrophages (PMf) phagocytic activity and Candida albicans (Ca) clearance after an ip challenge were analyzed. Cph administration induced a severe leukopenia with neutropenia. Lc431 and Lr06 groups showed a less severe neutropenia and an early recovery of blood neutrophils when compared with CG mice. In BM, Cph decreased the total number of cells affecting both the mitotic and post-mitotic pools. LAB treated groups showed higher numbers of mitotic pool cells and a less severe decrease in the total number of BM cells than CG mice (P