CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ADVANCES IN THE DESIGN OF A NOVELTY VETERINARIAN PRODUCT CONTAINING LACTOBACILLUS AND NATURAL SUBSTANCES TO IMPROVE THE PRODUCTIVE PERFOMANCE IN CATTLE
Autor/es:
M.C. OTERO; C.A. OVEJERO; M. E. NADER-MACÍAS
Lugar:
SAN MIGUEL DE TUCUMÁN
Reunión:
Congreso; VII CONGRESO ARGENTINO DE MICROBIOLOGIA; 2011
Institución organizadora:
SOCIEDAD ARGENTINA DE MICROBIOLOGÍA GENERAL
Resumen:
Metritis in cows reduces the reproductive efficiency, increases herd health costs, reduces food consumption and also causes an important reduction in milk production. Further, lower conception rate (extra inseminations are required), increased intervals from calving to conception and involuntary culling of cows. The probiotic lactobacilli therapy could be a valid alternative to the antibiotic application for the treatment of these infections. Four autochthonous strains: Lactobacillus gasseri CRL1412, Lactobacillus gasseri CRL1421, Lactobacillus gasseri CRL1460 and Lactobacillus delbrueckii subsp delbrueckii CRL1461 that exhibit beneficial properties (vaginal cells adhesion and bovine pathogens inhibition) that enable them to prevent metritis and technological properties, like ability to resist stress conditions during the freeze drying process and the shelf life period in two pharmaceutical forms. In this work, we tested the effects of natural substances (with active action on the impaired epithelium and its resident microbiota) on the growth kinetic of these microorganisms. A complete factorial experimental design was used to study the effect of three natural substances at different concentrations: Acid ascorbic (30,20 and 10 mg/mL), inulin (15,10 and 5 mg/mL) and Matricaria chamomilla extract (10 and 5 mg/mL) on the growth parameters of the four mentioned strains. The growth was tested in modified MRS medium at 37°C. The O.D. at 540 nm was determined at 30 min intervals during 14 h. And the increase of biomass, the maximal growth rates and the times of the lag and stationary phases were calculated. The experimental procedure was repeated three times following the complete factorial design. The maximal growth rate were significantly (p<0,005) affected by the presented substance in the culture medium, this effect was different, depending on the strain (interaction between strain x substance, p< 0,005). Being the inulin the substance that increased significantly this growth parameter, especially for the CRL 1412 strain. The increase of biomass was affected by the substance, but it response was depending on its concentration in the culture media and on the tested microorganisms (interaction strains x substance and substance x concentration, p<0,005), the major effect was observed on the CRL 1421 growth. The time of Lag phase was characteristic of the tested microorganisms; however it was not depending of the other factors. The time to stationary phase was significantly (p<0,05) shorted when microorganisms grew with inulin, in these conditions, CRL 1421 and CRL 1461 reached significantly earlier than other strains. The probiotic treatment success depends on the viability of the microorganisms included in the formula; during the shelf life and until they arrive at the target site in the animal. Therefore these results will allow the correct design of a probiotic product combining the microorganisms with natural substances to apply into bovine reproductive tract. This could be used in organic farmers or other safe production systems.