INTRANASAL ADDITION OF A PROBIOTIC BACTERIUM: EFFECT ON INFECTED MALNOURISHED MICE

ZELAYA H; HARO C; LAIÑO J; AGÜERO G

Tafi del Valle

Jornada; XXVII Jornadas Científicas de la Asociación de Biología de Tucumán.; 2010

Asociación de Biología de Tucumán.

Aim: to evaluate the effect of intranasal addition of Lactobacillus casei CRL 431 (Lc) on tissue damage and coagulation activation at lung level, in a respiratory infection model in malnourished mice. Malnourished mice (MN) received 7d balanced conventional diet (BCD) or BCD with intranasal addition of Lc during the last 2d (BCD+Lc). BCD, BCD+Lc, MN and Wellnourished (WN) groups were infected with Streptococcus pneumoniae. Results: Malnutrition altered all the parameters and renutrition with Lc normalized them, except Activated Protein C (APC). The infection induced an increase in Thrombin Antithrombin complexes (TATc) and a decrease in Antithrombin, APC, LDH activity and Albumin concentration in bronchoalveolar lavage. The pathogen was detected in lung and blood samples. However, BCD+Lc had negative hemocultures and significantly lower bacterial counts and lung damage, but they did not reach the TATc levels of WN (TATc24 h post infection MN= 5,25±0,90 µg/l; WN= 3,50±0,51; BCD= 3,50±0,45; BCD+Lc= 6,54±0,40). Conclusions: Lc intranasal addition with a renutrition diet contributes to pathogen depuration and to decrease lung damage. However, Lc did not influence on recovery of haemostatic parameters altered by challenge. It would be associated to dosis of Lc intranasal addition. It is convenient to evaluate other dosis of Lc to modulate coagulation in lung.