CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
Biochemical Charaterization of a Recombinant alpha-Galactosidase from Lactobacillus fermentum CRL 722
LEBLANC, J.G.; CARRERA-SILVA, E.A.; SESMA, F.; SAVOY DE GIORI, G.
Lugar: Mendoza, Argentina; Año: 2006 vol. 30 p. 172 - 172
The use of microbial alpha-galactosidase (alpha-Gal) which hydrolyzes alpha-1,6 galactoside links is a promising alternative to eliminate alpha-galactooligosaccharides (alpha-GOS) found in soy-derived products. These sugars often pass through the small intestine and reach the large intestine where they are fermented by the intestinal microbiota, producing gases that can cause physiological problems in sensitive individuals. The goal of this study was to characterize the alpha-Gal of Lb. fermentum CRL722 produced by E.coli. The alpha-Gal gene was amplified using degenerate primers, sequenced and cloned into E. coli. The recombinant enzyme was purified and compared to the alpha-Gal from the original strain (CRL722), both being active in a wide range of pH and temperatures and in the presence of various ions. The complete gene will now be amplified and inserted in probiotic microorganisms which would then be able to degrade alpha-GOS in situ in the gastrointestinal tract or use to produce novel fermented foods.