CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
artículos
Título:
Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells
Autor/es:
ZÁRATE, GABRIELA; PÉREZ CHAIA, ADRIANA
Revista:
JOURNAL OF APPLIED MICROBIOLOGY
Editorial:
Wiley-Blackwell Publishing
Referencias:
Año: 2009 vol. 106 p. 1050 - 1050
ISSN:
1364-5072
Resumen:
  ABSTRACT Aims: To assess in vitro the ability of some dairy bacteria to bind Concanvalin A (Con A), peanut agglutinin (PNA) and jacalin (AIL), preventing their toxicity on mouse intestinal epithelial cells (IEC). Methods and Results: Con A and AIL reduced significantly IEC viability in vitro,  as determined by Trypan  blue dye exclusion or by propidium iodide / fluorescein diacetate/ Hoescht staining. Different strains of dairy bacteria were able to remove lectins from the media. Two strains were subjected used to remove S-layer, cell wall proteins, polysaccharides and lectin-like adhesins. They were then assayed for the ability to bind dietary lectins and to reduce toxicity against IEC and to adhere to IEC after interaction with lectins. Con A and AIL were removed by Propionibacterium acidipropionici and Propionibacterium freudenreichii by binding with specific sugars moieties in the bacterial surface. Removal of lectins by bacteria impaired IEC protection. Adhesion of P. acidipropionici to IEC was reduced but not abolished after binding ConA or AIL. Conclusions: Removal of Con A or AIL by propionibacteria was effective to avoid the toxic effects against colonic cells in vitro. Significance and Impact of the Study: Consumption of food containing these bacteria would be a tool to protect intestinal epithelia.