Intestinal dysbiosis and reduced immunoglobulin-coated bacteria associated with coeliac disease in children

DE PALMA GIADA; INMACULADA NADAL; MEDINA MARCELA; DONAT ESTER; RIBES-KONINCKX CARMEN; MIGUEL CALABUIG; SANZ YOLANDA

BMC Microbiology

BIOMED CENTRAL

Año: 2010 vol. 24 p. 63 - 67

1471-2180

ABSTRACT Background: Celiac disease is a chronic intestinal inflammatory disorder due to an aberrant immune response to dietary gluten proteins in genetically predisposed individuals. Mucosal immune response through IgA secretion constitutes a first line of defence responsible for neutralizing noxious antigens and pathogens. The aim of this study was the characterization of the relationships between immunoglobulin-coated bacteria and bacterial composition in faeces of celiac disease (CD) patients, untreated and treated with a gluten-free diet (GFD) and healthy controls. Results: IgA-coated faecal bacterial levels were significantly lower in both untreated and treated CD patients than in healthy controls. IgG and IgM-coated bacterial levels were also significantly lower in treated CD patients than in untreated CD patients and controls. Gram-positive to Gram-negative bacteria ratio was significantly reduced in both CD patients compared to controls. Bifidobacterium, Clostridium histolyticum, C. lituseburense and Faecalibacterium prausnitzii group proportions were less abundant (P<0.050) in untreated CD patients than in healthy controls. Bacteroides-Prevotella group proportions were more abundant (P<0.050) in untreated CD patients than in controls. Levels of IgA coating the Bacteroides-Prevotella group were significantly reduced (P<0.050) in both CD patients in comparison with healthy controls. Conclusions: In CD patients, reduced IgA-coating bacteria is associated with intestinal dysbiosis, which altogether provide new insights into the possible relationships between the gut microbiota and the host defences in this disorder.