CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
Biofilms of vaginal Lactobacillus reuteri CRL 1324 and Lactobacillus rhamnosus CRL 1332: kinetics of formation and matrix characterization
RAULT L; NADER-MACÍAS MEF; JUÁREZ TOMÁS MS; EVEN S; LECCESE TERRAF MC; LE LOIR Y
ARCHIVES OF MICROBIOLOGY
Lugar: Berlin; Año: 2016 vol. 198 p. 689 - 689
Adhesion and biofilm formation are strain propertiesthat reportedly contribute to the permanence of lactobacilliin the human vagina. The kinetics of biofilm formationand the chemical nature of the biofilm matrix formedby Lactobacillus reuteri CRL (Centro de Referencia paraLactobacilos Culture Collection) 1324 and Lactobacillusrhamnosus CRL 1332, vaginal beneficial strains, wereevaluated in this work. Crystal violet-stained microplateassay and techniques of epifluorescence, electron and confocalmicroscopy were applied. The highest density andcomplexity of biofilms of both vaginal lactobacilli wereobserved at 72 h of incubation. Protease, proteinase K,α-chymotrypsin and trypsin treatments efficiently detachedL. reuteri CRL 1324 biofilm that was also partially affectedby α-amylase. However, L. rhamnosus CRL 1332 biofilmwas slightly affected by protease, proteinase K andα-amylase. Confocal microscopy revealed greater amountof polysaccharides in L. rhamnosus CRL 1332 biofilmmatrix than in L. reuteri CRL 1324 biofilm matrix. Theresults indicate that proteins are one of the main componentsof the L. reuteri CRL 1324 biofilm, while the biofilmmatrix of L. rhamnosus CRL 1332 is composed ofcarbohydrates and proteins. The results obtained supportthe knowledge, understanding and characterization of twobiofilm-forming vaginal Lactobacillus strains.