CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
Immunobiotic Lactobacillus strains reduce small intestinal injury induced by intraepithelial lymphocytes after Toll-like receptor 3 activation
TADA ASUKA; SALVA SUSANA; VILLENA JULIO; CLUA PATRICIA ; KITAZAWA HARUKI; ZELAYA HORTENSIA; ALVAREZ SUSANA
BIRKHAUSER VERLAG AG
Lugar: BASEL; Año: 2016
Objective Intestinal intraepithelial lymphocytes (IELs)play critical roles in disrupting epithelial homeostasis after Toll-like receptor (TLR)-3 activation with genomic rotavirus dsRNA or the synthetic dsRNA analog poly(I:C). The capacity of immunobiotic Lactobacillus rhamnosusCRL1505 (Lr1505) or Lactobacillus plantarum CRL1506(Lp1506) to beneficially modulate IELs response afterTLR3 activation was investigated in vivo using a mice model.Results Intraperitoneal administration of poly(I:C) induced inflammatory-mediated intestinal tissue damage through the increase of inflammatory cells (CD3+NK1.1+,CD3+CD8aa+, CD8aa+NKG2D+) and pro-inflammatory mediators (TNF-a, IL-1b, IFN-gamma, IL-15, RAE1, IL-8).Increased expression of intestinal TLR3, MDA5, and RIG-I was also observed after poly(I:C) challenge. Treatment with Lr1505 or Lp1506 prior to TLR3 activation significantly reduced the levels of TNF-a, IL-15, RAE1, and increased serum and intestinal IL-10. Moreover, CD3+-NK1.1+, CD3+CD8aa+, and CD8aa+NKG2D+ cells were+lower in lactobacilli-treated mice when compared to controls.The immunomodulatory capacities of lactobacilli+allowed a significant reduction of intestinal tissue damage. Conclusions This work demonstrates the reduction of TLR3-mediated intestinal tissue injury by immunobiotic lactobacilli through the modulation of intraepithelial lymphocytes response. It is a step forward in the understanding of the cellular mechanisms involved in the antiviral capabilities of immunobiotic strains.