CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
SCREENNING OF BENEFICIAL PROPERTIES OF LACTIC ACID BACTERIA ISOLATED FROM YOUNG CALVES FOR PROBIOTIC PURPOSES
NC MALDONADO, C SILVA DE RUIZ, M C OTERO, F SESMA, ME NADER-MACÍAS
Lugar: Amsterdan; Año: 2009
Lactic acid bacteria (LAB) are the dominant microorganisms in different human and animals tracts. They protect the host from the income of pathogenic microorganisms by different mechanisms. They have been widely used as probiotics to restore the ecological balance of different ecosystems, and also to help in the physiological functions of the host. Newborn calves are susceptible to diarrhoea, mainly because they do not receive antibodies from their mother during pregnancy. As the main interest of our research group is the design of a specie-specific probiotic for newborn calves. The isolation of 96 strains of LAB from faeces, oral cavity and colostrum of newborn calves was performed in this work, studying some beneficial properties. The surface properties of the isolated strain showed that they do not present hydrophobic characteristics, but some of them were able to autoagglutinate. Small amount of strains produced H202, but some others were able to inhibit diarrhoea pathogens by organic acid production. Only one bacteriocin-producer strain was detected. The functional properties assayed showed that some strains were able to grow at very lows pH and high bile salts concentrations, which make these strains potentially useful to be used as probiotics for the prevention of diarrhoea The strains sharing some properties were identified by genetic methodologies, to go further in the selection of a lower number of strains with probiotic characteristics, and later to test their efficacy through in vivo assays, to advance in the design of a specie-specific product for newborn calves.