Stability of salivaricin CRL 1328, a potential bioactive ingredient of urogenital probiotic products, after lyophilization and storage assessed by statistical methodology

VERA PINGITORE E.; BRU E.; NADER-MACIAS M.E.

JOURNAL OF APPLIED MICROBIOLOGY

Wiley-Blackwell

Lugar: Bedford; Año: 2009

1364-5072

Aims: To investigate the effects of different protectors on the activity of salivaricin CRL 1328, a bacteriocin produced by Lactobacillus salivarius CRL 1328, during lyophilization process and subsequent storage at different temperatures for 18 months through the application of classical and statistical models.Methods and Results: Some protectors [hexane-1,2,3,4,5,6-hexol (Mannitol), polysorbate 80 (Tween 80), polyethylenglycol 8000 (PEG), monosodium glutamate (MSG), reconstituted skim milk (RSM), sucrose and ascorbic acid] were used for the lyophilization process and storage of salivaricin. The principal components analysis biplot was used for the interpretation of the interactions between the different factors studied.Sucrose, mannitol + sucrose, PEG + sucrose and MSG were the most effective agents in protecting the bacteriocin during the lyophilization process. Effective maintenance of the bacteriocin activity was observed by storage with sucrose and ascorbic acid at -20ºC and PEG + sucrose at 4ºC and -20ºC Conclusions: The results suggest that PEG + sucrose and sucrose can effectively maintain the activity of salivaricin during lyophilization and storage. The ANOVA and the biplot graphics were useful tools to analyze and interpret the results obtained.Significance and Impact of the Study: This study provides useful information for the potential application of salivaricin as a bioactive principle for probiotic pharmaceutical formulation.(Aclaración: este trabajo ha sido enviado para su publicación).