Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis

KATRIEN DE BRUYNE; CHARLES M.A.P. FRANZ; MARC VANCANNEYT; ULRICH SCHILLINGER; FERNANDA MOZZI; GRACIELA FONT DE VALDEZ; LUC DE VUYST; PETER VANDAMME

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY.

Society for General Microbiology

Lugar: Reading, Inglaterra; Año: 2008 vol. 58 p. 2909 - 2916

1466-5026

A Gram-positive, small coccus-shaped lactic acid bacterium, strain LMG 23999T (CRL 776), was isolated from Argentinean wheat flour. 16S rRNA gene sequence analysis revealed its phylogenetic position within the genus Pediococcus, with Pediococcus stilesii, Pediococcus pentosaceus and Pediococcus acidilactici as closest relatives (97.7, 97.3 and 96.9% sequence similarity, respectively). Fluorescent amplified fragment length polymorphism fingerprinting of whole genomes and whole-cell protein electrophoresis confirmed its unique taxonomic status. DNA-DNA hybridizations, DNA G+C content determination, comparative sequence analysis of the genes pheS, rpoA and atpA, and physiological and biochemical characterization demonstrated that strain LMG 23999T (= CCUG 54535T) represents a new species for which the name Pediococcus argentinicus is proposed.