CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
Lactobacillus casei addition to a repletion diet induced early normalization of cytokine profiles during pneumococcal infection in malnourished mice
SALVA SUSANA; VILLENA JULIO; RACEDO SILVIA; AGÜERO GRACIELA; ALVAREZ SUSANA
FOOD AND AGRICULTURAL IMMUNOLOGY
Taylor & Francis
Lugar: London, England ; Año: 2008 vol. 19 p. 195 - 195
This work studied the influence of Lactobacillus casei on cytokine production during repletion of malnourished mice in the face of an infectious challenge. In addition, it was evaluated the number and function of cells involved in the immune response against a respiratory infection. Weaned mice were malnourished after consuming a protein-free diet (PFD) for 21d. Malnourished mice were fed a balanced conventional diet for 7 d (BCD) or BCD for 7 d with L. casei supplementation on d 6 and d 7 (BCD+Lc). The malnourished control group (MNC) received PFD while the well-nourished control mice (WNC) consumed BCD. Mice were challenged intranasally with Streptococcus pneumoniae at the end of each dietary treatment. Malnutrition impaired the levels of serum tumor necrosis factor (TNF)-a and interleukin (IL)-1b, IL-4, IL-6 and IL-10. In addition, neutrophil number and activity, lymphocyte maturation and bone marrow CD4+, CD8+ and CD19+ cells number, were also impaired in the MNC group. Repletion with BCD induced a slight improvement in some of the parameters studied. However, when L. casei was added to the BCD, it was observed a normalization of TNF-a, IL-1b and IL-6 values after infection and an increase of the levels of IL-10 and IL-4 compared to the WNC group. Moreover, BCD+Lc induced a significant improvement in blood and bone marrow cells. Consequently, the use of L. casei as a supplement in a repletion diet was associated with a pattern of inflammatory and anti-inflammatory cytokines that led to an increased number and functionality of the cells that participate in the immune response against a pneumococcal infection.