Characterization of the beneficial properties of lactobacilli isolated from bullfrog (Rana catesbeiana) hatchery

PASTERIS S.E.; VERA PINGITORE E.; ROIG BABOT G.; OTERO M.C.; BÜHLER M.I; NADER-MACIAS M.E.

ANTONIE VAN LEEUWENHOEK

Springer

Lugar: Wageningen; Año: 2009 vol. 95 p. 373 - 385

0003-6072

Abstract The present work addresses the isolationand partial identification of the microbial populationof a R. catesbeiana hatchery in spring and summer aswell as some beneficial properties of Lactobacillusstrains isolated in different seasons and hatcheryareas. The bacterial population was grouped into thefollowing taxa: Lactobacillus spp., Pediococcus spp.,Enterococcus faecalis and Ent. faecium, and Enterobacteriaceae(Enterobacter spp., Escherichia coli)while Pseudomonas aeruginosa and Staphylococcusepidermidis were isolated from frogs displaying redlegsyndrome. The Lactobacillus plantarum and L.curvatus strains isolated showed to inhibit the growthof red-leg syndrome associated pathogens and foodbornebacteria by organic acids. While L. plantarumCRL 1606 also inhibited red-leg syndrome relatedpathogens by hydrogen peroxide, meat spoilagebacteria were only inhibited by acidity. However,by using a MRS medium added with tetramethylbenzidineand peroxidase, a high percentage of H2O2-producing lactobacilli were detected. The surfaceproperties of Lactobacillus strains showed that a fewstrains were able to agglutinate ABO human erythrocytes,while the highest number of strains had a lowto medium degree of hydrophobicity. This paperconstitute the first study related to the beneficialproperties of Lactobacillus isolated from a bullfroghatchery, as well as the selection criteria applied to agroup of strains, which could help to control orprevent bacterial infectious diseases in raniculture.