CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
Production of bacteriocin-like inhibitory substances (BLIS) by Bifidobacterium lactis using milk whey as a substrate
BALCUINAS, E. M.; ARNI, S. A.; CONVERTI, A.; LEBLANC, J.G.; PINHEIRO DE SOUZA OLIVEIRA, R.
International Journal of Dairy Technology
Año: 2016 vol. 69 p. 236 - 236
The objective of this work was to evaluate the production of bacteriocin-like inhibitory substances (BLIS) by Bifidobacterium animalis subsp. lactis in milk whey supplemented with yeast extract, inulin, Tween 80 or L-cysteine. Cell growth, and acidification power, glucose and lactose consumption as well as BLIS production were investigated during along fermentations carried out in shake flasks. The best additive for both cell growth and BLIS production was shown to be yeast extract, whose supplementation to whey ensured the highest concentrations of biomass (9.9 log CFU/mL) and BLIS (200 AU/mL). In bench-scale fermenter, B. lactis growth and BLIS production were between about 6 and 25 % higher than in flasks, respectively depending on the conditions assayed.