MECHANISMS INDUCED BY LAB IN THE PREVENTION AGAINST SALMONELLA TYPHIMURIUM

GOBBATO, N; MALDONADO GALDEANO, C; PERDIGON, G.

FOOD AND AGRICULTURAL IMMUNOLOGY

Año: 2008 vol. 19 p. 11 - 23

0954-0105

ABSTRACT Objetives:To determine the possible mechanism induced by different lactic acid bacteria (LAB) in the protection against S. Typhimurium infection. Methods and results: The LAB was administered to BALB/c mice during different period of time and then they were challenged with S. Typhimurium. It was determined the inhibition of the S. TyphimuriumTo determine the possible mechanism induced by different lactic acid bacteria (LAB) in the protection against S. Typhimurium infection. Methods and results: The LAB was administered to BALB/c mice during different period of time and then they were challenged with S. Typhimurium. It was determined the inhibition of the S. TyphimuriumS. Typhimurium infection. Methods and results: The LAB was administered to BALB/c mice during different period of time and then they were challenged with S. Typhimurium. It was determined the inhibition of the S. TyphimuriumS. Typhimurium. It was determined the inhibition of the S. Typhimurium colonization in liver and the apoptosis in cut of the small intestine of mice fed previously with the LAB founding an decreased in the apoptosis mechanisms induced by the LAB assayed. It also it was analyzed the microbicidal activity of peritoneal macrophages, which was increased for L. delbr¨¹eckii subsp. bulgaricus and S. termophilus. The increase in the levels of IFN¦Ã and Bcl-2 positive cells in small intestine of mice fed with the LAB under study and in the control group were determined by immunofluorescence. Conclusion: The mechanisms induced by the LAB studied were different for each bacterium. We demonstrated by in vivo studies the immunomodulation exert by LAB on the gut mucosal immune response to prevent S. Typhimurium infection. It is the first in vivo approach in the understanding about the antiinfection effect mediated by LAB.L. delbr¨¹eckii subsp. bulgaricus and S. termophilus. The increase in the levels of IFN¦Ã and Bcl-2 positive cells in small intestine of mice fed with the LAB under study and in the control group were determined by immunofluorescence. Conclusion: The mechanisms induced by the LAB studied were different for each bacterium. We demonstrated by in vivo studies the immunomodulation exert by LAB on the gut mucosal immune response to prevent S. Typhimurium infection. It is the first in vivo approach in the understanding about the antiinfection effect mediated by LAB.Conclusion: The mechanisms induced by the LAB studied were different for each bacterium. We demonstrated by in vivo studies the immunomodulation exert by LAB on the gut mucosal immune response to prevent S. Typhimurium infection. It is the first in vivo approach in the understanding about the antiinfection effect mediated by LAB.in vivo studies the immunomodulation exert by LAB on the gut mucosal immune response to prevent S. Typhimurium infection. It is the first in vivo approach in the understanding about the antiinfection effect mediated by LAB.S. Typhimurium infection. It is the first in vivo approach in the understanding about the antiinfection effect mediated by LAB.infection. It is the first in vivo approach in the understanding about the antiinfection effect mediated by LAB.