Prospective uses of recombinant Lactococcus lactis expressing both Listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination

DE AZEVEDO, M.; SANTOS ROCHAT, C.; BASTOS PEREIRA, V.; DE OLIVEIRA, A.F.; DE SOUZA, V.; LEBLANC, J.G.; AZEVEDO, V.; CHATEL, J.M.; MIYOSHI, A.

GENETICS AND MOLECULAR RESEARCH

FUNPEC-EDITORA

Lugar: Ribeirao Preto San Pablo Brasil; Año: 2015 vol. 14 p. 18485 - 18493

1676-5680

In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with higher quantities found in the culture supernatants. A hemolytic assay showed that the recombinant strain secreted 250 ng active LLO/mg total protein. This mInlA/LLO-producing strain of L. lactis may be used as an alternative tool in DNA vaccination against a number of infectious diseases or in cancer therapy.