CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
STUDY OF SOME OF THE MECHANISMS INVOLVED IN THE PREVENTION AGAINST Salmonella enteritidis serovar Typhimurium INFECTION BY LACTIC ACID BACTERIA
GOBBATO, NADIA; MALDONADO GALDEANO, CAROLINA; PERDIGÓN, GABRIELA
FOOD AND AGRICULTURAL IMMUNOLOGY
Taylor & Francis
Lugar: London; Año: 2008 vol. 19 p. 11 - 11
We determined the possible mechanism exert by different lactic acid bacteria (LAB) in the protection against Salmonella enteritidis serovar Typhimurium (S. typhimurium) infection. LAB were administered to BALB/c mice; then, the animals were challenged with S. typhimurium. The inhibition of the translocation of the S. typhimurium in the liver was correlated with a decrease in the cellular apoptosis determined in slices from the small intestine of mice. The microbicidal activity of peritoneal macrophages was increased by Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus but not for the probiotic strain Lactobacillus casei CRL 431. The levels of IFNg and Bcl-2 positive cells in the small intestine of mice fed with these LAB were also determined by immunofluorescence. We demonstrated by in vivo studies that the biological and immune mechanisms induced by the LAB studied were different for each bacterium and they were mediated by antiS. typhimurium-S-IgA, microbiocidal activity and/or cellular apoptosis inhibition of infected immune cells.