CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
Lactobacillus casei CRL 431 administration decreases inflammatory cytokines in a diet induced obese mouse model
I. NOVOTNY NUÑEZ; C. MALDONADO GALDEANO; A. DE MORENO DE LEBLANC; G. PERDIGON
ELSEVIER SCIENCE INC
Lugar: Amsterdam; Año: 2015 vol. 31 p. 1000 - 1000
Objectives. Obesity is a chronic disease associated with an inflammatory process in which cytokines play an important role. Probiotic microorganisms have been associated with modulation of the host immune system. The aim of the present work was to evaluate the influence of the probiotic bacterium Lactobacillus (L.) casei CRL 431 on the cytokine response in a model of mice under high-fat diet. Methods. BALB/c mice received conventional-balanced-diet or high-fat-diet. The test groups received milk, milk fermented by L. casei (FM) or L. casei as suspension in the drinking water. Pro-inflammatory and regulatory cytokine producer cells were evaluated in the small intestine, liver and the cytokine levels in the intestinal fluids. The percentages of immune cell as macrophages (F4/80), NKT, CD4+, CD8+ populations were determined in the liver. Adipocytes were also isolated and cultured to evaluate cytokines and the chemokine MCP-1 produced by them. Results. The administration of probiotic L. casei CRL 431 exerted an anti-inflammatory response in mice under high-fat diet, evidenced mainly by decreasing pro-inflammatory cytokines, such as IL-6, IL-17 and TNF-α. Probiotic administration was also associated with less immune infiltrating cells in the liver of mice that received high-fat diet and decreased secretion of MCP-1 by the adipocytes. This last observation could be associated with less macrophage accumulation in the adipose tissues, which is characteristic in obese host and contributes to maintain the inflammatory response in this organ. The results obtained show an anti-inflammatory effect of L. casei CRL 431 when is administered as a supplement of the high-fat diet in a mouse model.