Optimization of the freeze-drying media and survival througout storage of freeze-dried Lactobacillus gasseri and L. delbrueckii for veerinarian probiotic application

MARIA CLAUDIA OTERO; CAROLINA ESPECHE; MARIA ELENA NADER-MACIAS

PROCESS BIOCHEMISTRY

Elsevier

Año: 2007 p. 1406 - 1411

0032-9592

The application of lactic acid bacteria (LAB) as vaginal probiotic cultures depends on the preservation technologies employed by the related industries. A full two-factor analysis of the variance, considering medium and strain, on the decrease of viability during freeze-drying was applied. Lactobacillus gasseri CRL1421 was significantly more resistant than L. gasseri CRL1412 to the process. L. gasseri CRL1412 resisted significantly more when was suspended in skim milk than in water, but lactose or sucrose did not increase significantly the viability after lyophilization. Lactobacillus delbrueckii subsp. delbrueckii CRL 1461, an autoaggregative strain, was significant more sensitive to the freeze-drying under the evaluated conditions.    The dried cultures were included in two pharmaceutical forms and the viability monitored during 270 days of storage. The optimal storage conditions was different for each microorganism, even though from the same specie.  Our results can be applied to the design of a veterinarian probiotic to prevent metritis in diary postpartum cows.