CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
artículos
Título:
Factors affecting the production of putrescine from
Autor/es:
ARENA M.E.; LANDETE J.M.; MANCA DE NADRA M. C.; PARDO I.; FERRER S.
Revista:
JOURNAL OF APPLIED MICROBIOLOGY
Referencias:
Año: 2008
ISSN:
1364-5072
Resumen:
Aims: To elucidate and characterize the metabolic putrescine synthesis pathway
from agmatine by Lactobacillus hilgardii X1B.To elucidate and characterize the metabolic putrescine synthesis pathway
from agmatine by Lactobacillus hilgardii X1B.Lactobacillus hilgardii X1B.
Methods and Results: The putrescine formation from agmatine by resting cells
(the normal physiological state in wine) of lactic acid bacteria isolated from
wine has been determined for the first time. Agmatine deiminase and N-carbamoylputrescine
hydrolase enzymes, determined by HPLC and LC-Ion Trap
Mass Spectrometry, carried out the putrescine synthesis from agmatine. The
influence of pH, temperature, organic acids, amino acids, sugars and ethanol
on the putrescine formation in wine was determined.The putrescine formation from agmatine by resting cells
(the normal physiological state in wine) of lactic acid bacteria isolated from
wine has been determined for the first time. Agmatine deiminase and N-carbamoylputrescine
hydrolase enzymes, determined by HPLC and LC-Ion Trap
Mass Spectrometry, carried out the putrescine synthesis from agmatine. The
influence of pH, temperature, organic acids, amino acids, sugars and ethanol
on the putrescine formation in wine was determined.N-carbamoylputrescine
hydrolase enzymes, determined by HPLC and LC-Ion Trap
Mass Spectrometry, carried out the putrescine synthesis from agmatine. The
influence of pH, temperature, organic acids, amino acids, sugars and ethanol
on the putrescine formation in wine was determined.
Conclusions: Resting cells of Lact. hilgardii X1B produce putrescine in wine.
The putrescine production was carried out from agmatine through the agmatine
deiminase system.Resting cells of Lact. hilgardii X1B produce putrescine in wine.
The putrescine production was carried out from agmatine through the agmatine
deiminase system.
Significance and Impact of the Study: These results have significance from two
points of view, wine quality and toxicological and microbiological aspects, taking
account that putrescine, which origin is still controversial, is quantitatively
the main biogenic amine found in wine.These results have significance from two
points of view, wine quality and toxicological and microbiological aspects, taking
account that putrescine, which origin is still controversial, is quantitatively
the main biogenic amine found in wine.