CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
artículos
Título:
ARGININE METABOLISM IN WINE Lactobacillus plantarum: IN VITRO ACTIVITIES OF THE ENZYMES ARGININE DEIMINASE (ADI) AND ORNITHINE TRANSCARBAMILASE (OTCASE).
Autor/es:
SPANO, G.; MASSA, S; ARENA, M.E.; MANCA DE NADRA M. C
Revista:
Annals of Microbiology
Referencias:
Lugar: Italia; Año: 2007 vol. 57 p. 67 - 70
ISSN:
1590-4261
Resumen:
This work was carried
out to determine the activity of enzymes involved in arginine metabolism in Lactobacillus
plantarum isolated from wine and previously characterised at molecular
level. The activity of the enzymes arginine deiminase and ornithine
tran-scarbamylase was determined and citrulline and ornithine formed were
analysed by HPLC analysis. Although the enzymatic activity was detected in all
the strains analysed, a strong variability was observed between strains. Lactobacillus
plantarum strain Lp60 is the strain with more possibilities to accumulate
citrulline, precursor of the carcinogenic ethyl-carbamate, as showed by its
high arginine deiminase activity and low ornithine transcarbamylase activity.