CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
artículos
Título:
Killer activity of Saccharomyces cerevisiae strains: partial characterization and strategies to improve the biocontrol efficacy in winemaking
Autor/es:
LUCÍA M. MENDOZA; RAÚL R. RAYA; MIGUEL FERNÁNDEZ DE ULLIVARRI
Revista:
ANTON LEEUW INT. J. G.
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2014 vol. 106 p. 865 - 878
ISSN:
0003-6072
Resumen:
Killer yeasts are considered potential biocontrol agents to avoid or
reduce wine spoilage by undesirable species. In this study two Saccharomyces cerevisiae (Cf8 and M12) strains
producing killer toxin were partially characterized and new strategies to improve
their activity in winemaking were evaluated. Killer toxins were characterized
by biochemical tests and growth inhibition of sensitive yeasts. Also genes
encoding killer toxin were detected in chromosome of both strains by PCR. Both
toxins showed optimal activity and production at conditions used during the
wine-making process (pH 3.5 and temperatures of 15-25 °C). In addition,
production of both toxins was higher when a nitrogen source was added. To
improve killer activity different strategies of inoculation were studied, being
the sequential inoculation of killer strains the best combination to control
the growth of undesired yeasts. Sequential inoculation of Cf8-M12 showed a 45 %
increase of killer activity on sensitive S.cerevisiae and spoilage yeasts. In
presence of ethanol (5-12%) and SO2 (50 mg/L) the killer activity of
both toxins was increased, especially for toxin Cf8. Characteristics of both
killer strains support their future application as starter cultures and biocontrol
agents to produce wines of controlled quality.