CERELA 05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
artículos
Título:
Gastroprotection as an example:Antiadhesionagainst Helicobacter pylori, anti-inflammatoryand antioxidant activities of aqueous extracts from the aerial parts of Lippia integrifolia Hieron
Autor/es:
MARCIAL G.; SENDKER J.; BRANDT S.; PEROTTI M; CATALÁN C.; FONT DE VALDEZ, G.; HENSEL, A.
Revista:
JOURNAL OF ETHNOPHARMACOLOGY
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 155 p. 1125 - 1133
ISSN:
0378-8741
Resumen:
Ethnopharmacological relevance: The aerialpartsof Lippia integrifolia (Gris.) Hieronymus(Verbenaceae),known as incayuyo, reusedbythepeasantpopulationofArgentinafortreatmentofdiseasesrelatedtoa gastrointestinalsystem,mainlyforinflammation ofthestomachandhavealsobeenincludedintothe Argentina FoodCode.Thisstudyaimedtoinvestigatethephytochemicalprofile ofhydrophilicextracts from theherbalmaterialbyLCMS andtoevaluatepotentialpharmacologicalmechanismsrationalizing the traditionaluseof incayuyo aqueousextractspotentialanti-inflammatorytreatmentofgastrointest-inal disorders. Materials andmethods: Phytochemicalprofiling: LCMS fanaqueousdecoction.Antiadhesiveeffects against Helicobacter pylori: in vitro FACSassayusingFITC-labeledbacteriaandAGShumanstomachcells. Influence ofextractsonstomachcellsandRAW264.7macrophages:MTTviabilityassayandBrdU proliferationELISA.Influence ofextractsonIL-6andIL-8secretionfromstomachcellswasquantitatedby ELISA afterinfectionofthecellswith Helicobacter pylori. Influence oftestextractsonmacrophages:phagocytosisofFITC-labeledZymosanparticlesandNOproduction.Antioxidativecapacity:DPPHassay and O2-induced carotenoxidation. Results: LCMS profiling indicatedthepresenceofcompounds 120 with flavonoidhexosides, phenylethanoides(acteoside,isoacteoside)andsesquiterpenes[(epi)lippidulcine,peroxylippidulcine]in hedecoctionextractandsubfractionPhF.Theextractexhibitsstrong in vitro antioxidativecapacity and inhibitedadhesionof Helicobacter pylori to stomachcellsupto40%,whileanEtOH-solublefraction showedinhibitionratesofupto60%.DecoctionincreasedthecellularviabilityofAGScellssignificantly at 410 μg/mL, whileproliferationratewasnotinfluenced. Helicobacter pylori induced IL-8secretionwas significantly reducedbycoincubationofAGScellswiththeextracts.Aqueousextractsstimulated phagocytosisrateofmacrophagesandinhibitedtheLPS-inducedNO-secretion. Conclusions: The traditionaluseofaqueousextractsfrom Lippia integrifolia for gastricinflammation seems toberationalized:besidesanti-inflammatory effectsonstomachcellsantiadhesiveproperties of the extractsagainstthemainbacterialinductorofgastritis Helicobacter pylori wereidentified. Additional effects forstimulationofinnateimmunityandpotentialradicalscavengingeffectsmayadditionally contribute totheactivityoftheextracts.
