Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef.

CECILIA FONTANA; PIER SANDRO COCCONCELLI; GRACIELA VIGNOLO

APPLIED AND ENVIRONMENTAL MICROBIOLOGY

American Society for Microbiology

Lugar: Washington; Año: 2006 vol. 72 p. 5618 - 5622

0099-2240

DGGE allowed us to monitor total bacterial communities and to establish a pattern of succession between species in vacuum-packaged beef stored at 2 and 8ºC for 9 weeks and 14 days, respectively. Species-specific PCR was used to confirm the presence of L. sakei and L. curvatus. Multiplex PCR reactions using 16S rRNA specific primers allowed differentiation between Leuconostoc species. These methods provided the desired information about microbial diversity by detecting the main microorganisms capable of colonizing this ecological niche.