CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
artículos
Título:
Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef.
Autor/es:
CECILIA FONTANA; PIER SANDRO COCCONCELLI; GRACIELA VIGNOLO
Revista:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Editorial:
American Society for Microbiology
Referencias:
Lugar: Washington; Año: 2006 vol. 72 p. 5618 - 5618
ISSN:
0099-2240
Resumen:
DGGE allowed us to monitor total bacterial communities and to establish a pattern of succession between species in vacuum-packaged beef stored at 2 and 8ÂșC for 9 weeks and 14 days, respectively. Species-specific PCR was used to confirm the presence of L. sakei and L. curvatus. Multiplex PCR reactions using 16S rRNA specific primers allowed differentiation between Leuconostoc species. These methods provided the desired information about microbial diversity by detecting the main microorganisms capable of colonizing this ecological niche.