CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
artículos
Título:
Study of immune cells involved in the antitumor effect of kefir in a murine breast cancer model
Autor/es:
A. DE MORENO DE LEBLANC; C. MATAR; E. FARNWORTH; G. PERDIGON
Revista:
Journal of Dairy Science
Editorial:
American Dairy Science Association
Referencias:
Año: 2007 vol. 90 p. 1920 - 1928
ISSN:
0022-0302
Resumen:
Administration of kefir and a kefir cell-free fraction
(KF) to mice injected with breast tumor cells produced,
locally in the mammary gland, different profiles of cells
secreting cytokines. Here, the immune cell populations
in mammary glands affected by the cyclic consumption
of kefir or KF for 2 or 7 d were evaluated using a breast
tumor model. Apoptosis was also assayed as another
mechanism involved in tumor growth delay. The rate
development of tumor cells, IgA(+) cells, and CD4+ and
CD8+ T lymphocytes was monitored in mammary gland
tissues. The number of Bcl-2(+) cells in the mammary
gland was compared with the apoptosis observed in the
tumor. Two-day cyclical administration of both products
delayed tumor growth and increased the number
of IgA(+) cells in the mammary gland. Changes in the
balance between CD4+ and CD8+ cells in the mammary
gland were observed in mice from the group fed KF
cyclically for 2 d, such that the number of CD4+ cells
increased when the number of CD8+ cells remained
constant. Mice that received 2-d cyclic administration
of KF showed significant increases in the number of
apoptotic cells and decreases in Bcl-2(+) cells in the
mammary gland, compared with the tumor control
group. The present study allows a better understanding
of the mechanisms (immune and nonimmune) involved
in the antitumor effect observed in mice administered
kefir or KF. The importance of nonmicrobial components
released during milk fermentation to obtain the
beneficial antitumor effects is also reported.