CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
artículos
Título:
Milk fermented by Lactobacillus helveticus R389 and its non-bacterial fraction confer enhanced protection against Salmonella enteritidis serovar Typhimurium infection in mice
Autor/es:
VINDEROLA, CELSO GABRIEL; MATAR, CHANTAL; PERDIGON, GABRIELA
Revista:
IMMUNOBIOLOGY.
Referencias:
Año: 2006 vol. 212
ISSN:
0171-2985
Resumen:
Bacterial infections in the gastrointestinal tract represent a major global health problem, even in the presence of
normally effective mucosal immune mechanisms. Milk fermented by Lactobacillus helveticus R389 (FM) or its nonbacterial
fraction obtained by milk fermentation at controlled pH 6 (NBF) are able to activate the small intestine
mucosal immune response according to previous studies. In this work we aimed at comparing their protection capacity
against an infection by Salmonella enteritidis serovar Typhimurium and at studying the mechanisms involved. In a
completely randomized design, BALB/c mice received FM or NBF for 2, 5 or 7 consecutive days, followed by a single
oral challenge with S. Typhimurium (107 cells/mouse). The increase in the number of IgA+ cells in the lamina propria
of the small intestine, after the feeding periods, was accompanied by an increase in the luminal content of total S-IgA.
However, no antibodies were produced against the NBF. In mice given the FM or the NBF for 7 consecutive days,
lower levels of liver colonization on day 7 post-challenge with S. Typhimurium, higher luminal contents of specific
anti-Salmonella S-IgA, higher percentages of survival to infection and lower numbers of MIP-1a+ cells in the lamina
propria were observed. In this work we observed that in both the FM or the NBF there are active principles that confer
enhanced protection against S. Typhimurium infection. However, the mechanisms underlying mucosal immunomodulation
and protection are different. In those mechanisms, the mucosal immune response would seem to be more
involved than the competitive or exclusion mechanisms between L. helveticus R389 and S. enteritidis serovar
Typhimurium.Lactobacillus helveticus R389 (FM) or its nonbacterial
fraction obtained by milk fermentation at controlled pH 6 (NBF) are able to activate the small intestine
mucosal immune response according to previous studies. In this work we aimed at comparing their protection capacity
against an infection by Salmonella enteritidis serovar Typhimurium and at studying the mechanisms involved. In a
completely randomized design, BALB/c mice received FM or NBF for 2, 5 or 7 consecutive days, followed by a single
oral challenge with S. Typhimurium (107 cells/mouse). The increase in the number of IgA+ cells in the lamina propria
of the small intestine, after the feeding periods, was accompanied by an increase in the luminal content of total S-IgA.
However, no antibodies were produced against the NBF. In mice given the FM or the NBF for 7 consecutive days,
lower levels of liver colonization on day 7 post-challenge with S. Typhimurium, higher luminal contents of specific
anti-Salmonella S-IgA, higher percentages of survival to infection and lower numbers of MIP-1a+ cells in the lamina
propria were observed. In this work we observed that in both the FM or the NBF there are active principles that confer
enhanced protection against S. Typhimurium infection. However, the mechanisms underlying mucosal immunomodulation
and protection are different. In those mechanisms, the mucosal immune response would seem to be more
involved than the competitive or exclusion mechanisms between L. helveticus R389 and S. enteritidis serovar
Typhimurium.Salmonella enteritidis serovar Typhimurium and at studying the mechanisms involved. In a
completely randomized design, BALB/c mice received FM or NBF for 2, 5 or 7 consecutive days, followed by a single
oral challenge with S. Typhimurium (107 cells/mouse). The increase in the number of IgA+ cells in the lamina propria
of the small intestine, after the feeding periods, was accompanied by an increase in the luminal content of total S-IgA.
However, no antibodies were produced against the NBF. In mice given the FM or the NBF for 7 consecutive days,
lower levels of liver colonization on day 7 post-challenge with S. Typhimurium, higher luminal contents of specific
anti-Salmonella S-IgA, higher percentages of survival to infection and lower numbers of MIP-1a+ cells in the lamina
propria were observed. In this work we observed that in both the FM or the NBF there are active principles that confer
enhanced protection against S. Typhimurium infection. However, the mechanisms underlying mucosal immunomodulation
and protection are different. In those mechanisms, the mucosal immune response would seem to be more
involved than the competitive or exclusion mechanisms between L. helveticus R389 and S. enteritidis serovar
Typhimurium.S. Typhimurium (107 cells/mouse). The increase in the number of IgA+ cells in the lamina propria
of the small intestine, after the feeding periods, was accompanied by an increase in the luminal content of total S-IgA.
However, no antibodies were produced against the NBF. In mice given the FM or the NBF for 7 consecutive days,
lower levels of liver colonization on day 7 post-challenge with S. Typhimurium, higher luminal contents of specific
anti-Salmonella S-IgA, higher percentages of survival to infection and lower numbers of MIP-1a+ cells in the lamina
propria were observed. In this work we observed that in both the FM or the NBF there are active principles that confer
enhanced protection against S. Typhimurium infection. However, the mechanisms underlying mucosal immunomodulation
and protection are different. In those mechanisms, the mucosal immune response would seem to be more
involved than the competitive or exclusion mechanisms between L. helveticus R389 and S. enteritidis serovar
Typhimurium.S. Typhimurium, higher luminal contents of specific
anti-Salmonella S-IgA, higher percentages of survival to infection and lower numbers of MIP-1a+ cells in the lamina
propria were observed. In this work we observed that in both the FM or the NBF there are active principles that confer
enhanced protection against S. Typhimurium infection. However, the mechanisms underlying mucosal immunomodulation
and protection are different. In those mechanisms, the mucosal immune response would seem to be more
involved than the competitive or exclusion mechanisms between L. helveticus R389 and S. enteritidis serovar
Typhimurium.Salmonella S-IgA, higher percentages of survival to infection and lower numbers of MIP-1a+ cells in the lamina
propria were observed. In this work we observed that in both the FM or the NBF there are active principles that confer
enhanced protection against S. Typhimurium infection. However, the mechanisms underlying mucosal immunomodulation
and protection are different. In those mechanisms, the mucosal immune response would seem to be more
involved than the competitive or exclusion mechanisms between L. helveticus R389 and S. enteritidis serovar
Typhimurium.S. Typhimurium infection. However, the mechanisms underlying mucosal immunomodulation
and protection are different. In those mechanisms, the mucosal immune response would seem to be more
involved than the competitive or exclusion mechanisms between L. helveticus R389 and S. enteritidis serovar
Typhimurium.L. helveticus R389 and S. enteritidis serovar
Typhimurium.